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一种烧结烟气氨法脱硫工艺产生的硫铵母液净化的方法
, , 1900 ,
本发明涉及一种烧结烟气氨法脱硫工艺产生的硫铵母液净化的方法。其特征在于：它是在10-45℃，向硫铵母液中加入无机高分子絮凝剂聚合氯化铝或聚合硫酸铝，搅拌分散，然后加入改善絮凝体结构的高分子助凝剂，搅拌分散，再静置充分沉淀，收集上层清液即处理后的硫铵母液，然后将下层沉淀物用板框式压滤机压滤，收集压滤得到的滤液即处理后的硫铵母液，并与前述收集的上层清液合并。该工艺简单，净化效果好；经处理后的硫铵母液体系中基本无絮凝剂和助凝剂残留，因此将其返回至脱硫塔重复使用时不会对脱硫塔内的工艺带来不利影响；成本低廉，便于推广。
氢气纳米探针及氢气检测方法
, , 1900 ,
本发明公开一种氢气纳米探针及氢气检测方法，所述氢气纳米探针包括介孔载体、贵金属纳米颗粒以及与氢气反应的探针分子，所述贵金属纳米颗粒负载在所述介孔载体上，所述探针分子负载在所述介孔载体上，所述探针分子中含有能与氢气反应的官能团。本发明提供的氢气纳米探针，采用介孔载体作为负载贵金属纳米颗粒和荧光分子的载体，有利于氢气纳米探针的生物安全性，提高其在生物内的适用性；而探针分子中的官能团能够被氢气还原，从而出现吸电子能力的变化，实现对氢气的检测；而贵金属纳米颗粒的存在会促进氢气的催化还原，提高氢气检测效率；该氢气纳米探针有助于对氢气治疗提供一个有效检测手段。
一种聚集体材料功能信息的数据交互系统和方法
, , 1900 ,
本发明提供一种聚集体材料功能信息的数据交互系统和方法。所述系统包括核心数据库，用于临时储存新录入系统的材料条目、存储分子所处的不同聚集状态以及各聚集状态下的光物理性质；数据库查询系统，能够根据材料在应用中的不同聚集状态进行检索，用于根据字段值或分子的化学结构在核心数据库中进行检索，并将搜索字段和值返回数据库后端，经后端处理后返回其相关的所有信息并生成搜索报告页；数据更新系统，用于在核心数据库中录入新的材料条目；机器学习API系统，用于输出符合搜索条件的条目。本发明能够将材料归属于材料的不同聚集状态，能够克服传统材料和分子数据库中无法针对材料的不同状态储存、分类、索引和查询数据的不足。
一种荧光探针成像性能的评估方法及系统
, , 1900 ,
本发明提供一种荧光探针成像性能的评估方法及系统。所述评估方法包括如下步骤：(1)对荧光探针的通用功能、专业功能和补充功能分别进行评分，得到通用功能的分值、专业功能的分值和补充功能的分值；(2)对步骤(1)得到的通用功能的分值、专业功能的分值和补充功能的分值进行分析得到总分，完成所述荧光探针成像性能的评估。本发明通过对荧光探针的不同功能参数进行评分、分析，客观、全面的完成了荧光探针成像性能的评估，解决了现有技术中只能通过比较荧光探针的单一参数，无法全面反映荧光探针成像性能的问题。
一种检测细菌的方法
, , 1900 ,
本发明公开了一种检测细菌的方法。本发明将MASPB荧光探针与含有细菌的待测溶液混合，静置得混合溶液；混合溶液在紫外光下检测待测溶液中细菌的种类或浓度；所述细菌为革兰氏阳性菌和革兰氏阴性菌中的至少一种；所述MASPB荧光探针的结构为：#imgabs0#本发明的MASPB荧光探针与不同的菌种具有不同的结合位点，能够进行肉眼和细胞水平上对不同细菌进行区分，实现快速、准确的对革兰氏阳性菌和革兰氏阴性菌的区分，荧光强度与细菌量呈现良好的线性关系，可以检测细菌的浓度，实用性较高。
一种快速检测细菌浓度的方法
, , 1900 ,
本发明公开了一种快速检测细菌浓度的方法；本发明将Car‑py荧光探针与含有细菌的待测溶液混合，静置得混合溶液；检测混合溶液在紫外光下的荧光强度，得到细菌的浓度；所述Car‑py荧光探针的结构为：本发明基于AIE荧光探针(Car‑py)与多种细菌结合引起的聚集诱导发光特性，对细菌具有灵敏的荧光响应，其聚集发光效率高，光稳定性好，荧光强度与细菌量成正比，并且该检测过程操作简单，无需进行细菌培养阶段，耗时短，对检测样本形式的包容性较强，能实现快速、准确的细菌浓度检测，实用性高。
一种水溶性近红外AIE聚合物纳米粒子的制备方法和应用
, , 1900 ,
本发明公开一种水溶性近红外AIE聚合物纳米粒子的制备方法和应用。制备方法包括以下步骤：将AIE分子和水溶性高分子聚合物溶于与水互溶的挥发性有机溶剂中，得到有机相溶液；在室温下，将有机相溶液在超声和混合搅拌条件下加至去离子水溶液中，超声结束后，挥发性有机溶剂在避光通风条件下除去，得到AIE聚合物纳米粒子溶液，过滤得到水溶性近红外AIE聚合物纳米粒子。本发明制备的水溶性近红外AIE聚合物纳米粒子具有粒径均一、光稳定性好、重复性好、可规模化生产等优点，在生物活体荧光成像领域有良好的应用前景。
一种AIE荧光纳米颗粒制备系统
, , 1900 ,
本申请提出一种AIE荧光纳米颗粒制备系统，包括第一储液罐和第二储液罐、第一反应容器、第二反应容器、第三反应容器，所述第一反应容器、所述第二反应容器外套设有超声波换能器，所述第三反应容器内设置有超声波探头；第三反应容器还设置有磁力搅拌装置，其中，第一反应容器的容积大于第二反应容器的容积；所述超声波探头伸入所述第三反应容器内部；连通管组件，连通管组件连通储液罐组件、反应容器组件，以形成第一流路、第二流路、第三流路，其中，第一流路依次连通第一储液罐、第一反应容器、第三反应容器，第二流路依次连通第一储液罐、第二反应容器、第三反应容器；第三流路依次连通第二储液罐、第三反应容器。如此，本申请解决了纳米颗粒生产中精度难以控制和自动化生产的问题。
一种白细胞检测试剂盒及其应用
, , 1900 ,
本发明公开了一种白细胞检测试剂盒及其应用；所述白细胞检测试剂盒包括式（1）到式（4）中任意一种结构的AIE荧光染料。本发明将血液样品和红细胞溶解剂混合后，加入AIE荧光染料溶液；检测所得混合物的散射光特性和荧光特性；根据散射光特性和荧光特性对白细胞进行分类和计数。本发明利用具有聚集诱导发光特性的AIE荧光染料作为白细胞分类试剂，AIE荧光染料特异性结合白细胞的核酸后，通过流式细胞仪检测，可以对白细胞进行准确的分类统计；而且AIE荧光染料检测灵敏性高，光稳定性好，可降低单次使用剂量，不易发生荧光猝灭现象。
一种基于聚集诱导发光材料的核酸凝胶染料及其应用
, , 1900 ,
本发明公开了一种基于聚集诱导发光材料的核酸凝胶染料及其应用，所述基于聚集诱导发光材料的核酸凝胶染料包括式(1)、式(2)中任意一种结构的AIE分子。本发明基于聚集诱导发光材料的核酸凝胶染料用于核酸电泳常见的凝胶成像染色，可同时适用于琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳，尤其可用蓝光成像。本发明的基于聚集诱导发光材料的核酸凝胶染料，集染色效果好、灵敏度高、染色时间短、以及适应现有的所有染料激发通道，成为与激光扫描仪配合使用的理想选择。有效解决了现有核酸染料，由于染色效果差、应用在聚丙烯酰胺凝胶电泳时，灵敏度较低的缺陷，尤其解决了现有染料激发通道比较单一等问题。
一种AIE阴道分泌物双重荧光染色液及其应用
, , 1900 ,
本发明公开了一种AIE阴道分泌物双重荧光染色液及其应用；所述AIE阴道分泌物双重荧光染色液包括荧光剂；所述荧光剂包括第一荧光剂和第二荧光剂；第一荧光剂与第二荧光剂的摩尔比为1：（1‑6）。本发明AIE阴道分泌物双重荧光染色液中的第一荧光剂和第二荧光剂能够协同作用，实现双重染色的效果，并且能增加染色的强度和清晰度，提高染色效果，进而有助于增加检测的稳定性和灵敏度；两种荧光剂都是AIE荧光染料，具有背景低和光稳定性好的优势，无需添加其他辅助染料和抗猝灭剂，成分简单，成本低。
一种快速标记蛋白的AIE荧光试剂及其应用
, , 1900 ,
本发明公开了一种快速标记蛋白的AIE荧光试剂及其应用；所述AIE荧光试剂包括AIE荧光分子；所述AIE荧光分子包括结构通式（1）所示AIE荧光分子中的至少一种。本发明将待标记蛋白质加入所述的AIE荧光试剂中反应，反应结束后，使用过滤器过滤得到滤液，即得AIE荧光分子标记的蛋白质；其中，所述过滤器的孔径为0.05~0.1μm。本发明快速标记蛋白的AIE荧光试剂标记蛋白质的反应时间短、反应条件温和、后续的纯化操作也非常简便。
一种AIE纳米颗粒制备用搅拌装置
, , 1900 ,
本实用新型公开了一种AIE纳米颗粒制备用搅拌装置，本实用新型涉及AIE纳米颗粒制备技术领域，包括搅拌容器和超声波分散器，所述超声波分散器的前端设置有隔音门，所述超声波分散器内侧的下端设置有升降座，所述搅拌容器位于超声波分散器的内侧，且和升降座的上端相互适配，所述超声波分散器内侧的上端设置有升降盖，所述升降盖的下端设置有升降环，所述升降环上端的前侧固定安装有电机仓，所述升降环的内侧设置有齿轮，所述升降环的下端设置有搅拌叶，所述升降盖的下端设置有超声波输出探头。该AIE纳米颗粒制备用搅拌装置，通过驱动电机工作带动多个搅拌叶同时转动，实现对溶液的充分搅拌混合，通过超声波输出探头实现对纳米颗粒的充分分散。
一种快速检测抗菌药物敏感性的AIE试剂及方法
, , 1900 ,
本发明公开了一种快速检测抗菌药物敏感性的AIE试剂及方法。本发明AIE分子包括AIE‑1分子，优选的还包括AIE‑2分子，用于体外抗菌药物敏感性检测。所述AIE试剂的灵敏度高、荧光背景低且光稳定性好，可以直接将试剂加入药敏测试稀释液中，实时进行细菌生长的长时间监测，通过细菌生长曲线，快速得出药敏结果且满足MIC值测定的需求。本发明的快速检测抗菌药物敏感性的方法，能够在8h内获取药敏试验结果，且准确判断抗菌药物的MIC值，适用于不同的培养基，且检测设备的要求低，大大提高了体外抗菌药物敏感性试验的效率，能够为抗菌治疗快速提供解决方案。
一种AIE荧光分子在实时荧光定量PCR或高分辨率熔解曲线分析中的应用
, , 1900 ,
本发明公开了一种AIE荧光分子在实时荧光定量PCR或高分辨率熔解曲线分析中的应用；所述AIE荧光分子选自式(1)、式(2)结构中的任意一种。本发明AIE荧光分子用于实时荧光定量PCR和高分辨率熔解曲线分析，集灵敏度高、抗抑制剂能力强、分辨率高、多重兼容等优势，是通过高分辨率熔解曲线进行基因分型以及病原体多重检测时使用的理想染料。有效解决了现有核酸染料，由于灵敏度差、抗抑制剂能力差，应用在较低浓度模板样本及复杂样本时，效果不佳或无法检测的缺陷，尤其解决了现有染料无法提供每个序列的特异性信息等问题。
一种基于AIE荧光探针的抗酸染色液及其应用
, , 1900 ,
本发明公开一种基于AIE荧光探针的抗酸染色液及其应用；本发明的抗酸染色液包括AIE染色液和酸性脱色液；所述AIE染色液包括MASPB分子和水，MASPB分子的结构式如下：#imgabs0#。本发明的抗酸染色液用于分枝杆菌的检测，准确率高、抗干扰性强、时间短。
一种基于AIE荧光染料的白细胞分类试剂及其制备方法和应用
, , 1900 ,
本发明公开了一种基于AIE荧光染料的白细胞分类试剂及其制备方法和应用；本发明的白细胞分类试剂包括以下组分：AIE荧光染料、表面活性剂、芳香族有机酸和水；其中，所述表面活性剂包括质量比为0.5～5：1的非离子表面活性剂和阳离子表面活性剂，或质量比为0.5～20：1的非离子表面活性剂和阴离子表面活性剂。本发明的白细胞分类试剂兼具红细胞裂解功能和白细胞荧光染色功能，并具有良好的储存稳定性，仅采用单一试剂即可实现对血液样本的预处理，极大地简化了白细胞分类与计数分析的血液试样预处理操作流程，有效减少了测试误差，提高检测结果的可靠性。
一种曲妥珠单抗修饰的AIE荧光纳米颗粒及其制备方法和应用
, , 1900 ,
本发明公开了一种曲妥珠单抗修饰的AIE荧光纳米颗粒及其制备方法和应用；本发明的曲妥珠单抗修饰的AIE荧光纳米颗粒由AIE荧光纳米颗粒和曲妥珠单抗偶联得到，所述AIE荧光纳米颗粒表面含有羧基和氨基中的至少一种，所述AIE荧光纳米颗粒内部负载有AIE分子；曲妥珠单抗修饰的AIE荧光纳米颗粒的平均粒径为50‑300nm，分散系数为0.05‑0.25。本发明的曲妥珠单抗修饰的AIE荧光纳米颗粒用于乳腺癌肿瘤荧光靶向，在乳腺癌手术导航的过程中能够有效靶向乳腺癌肿瘤，实时成像，获得高信噪比和分辨率的图像，精准指示乳腺癌的病灶及边缘。
一种白细胞分类检测用溶血剂和应用
, , 1900 ,
本发明公开了一种白细胞分类检测用溶血剂和应用；本发明的白细胞分类检测用溶血剂包括表面活性剂、有机酸和水；所述有机酸选自芳香族有机酸及其衍生物中的任意一种或多种；所述表面活性剂为非离子表面活性剂和阴离子表面活性剂复配，或者非离子表面活性剂和两性离子表面活性剂复配，或者非离子表面活性剂和阳离子表面活性剂复配。本发明的溶血剂可以与AIE荧光染料混合使用，不会产生干扰或相互作用，能够高效裂解红细胞，同时最大程度地保留白细胞的形态特征和内部结构，且对白细胞DNA的响应灵敏度高，实现白细胞细胞核特异性标记，有效提高白细胞分类检测中对血液样本的处理效率，以及保证样本处理的可靠性和检测结果的准确性。
一种药敏检测方法
, , 1900 ,
本发明公开了一种药敏检测方法，包括设置微孔板、培养微孔板和定时收集荧光信号并分析数据。本发明基于细菌生长特点，利用荧光判读的优势，实现实时监测分析，能更快的获取药敏结果，及时终止监测，时间短、效率高；并且对设备要求低，用常用的具有发光检测功能的多功能读板机就能实现监测分析。
一种AIE分子标记的噬菌体及其制备方法与应用
, , 1900 ,
本发明公开一种AIE分子标记的噬菌体及其制备方法与应用；本发明的AIE分子标记的噬菌体包括MASPB和噬菌体，MASPB与噬菌体内部的遗传物质结合。本发明的MASPB可以进入噬菌体内部对噬菌体的遗传物质进行标记，形成AIE分子标记的噬菌体，从而对目标细菌进行快速准确地检测。
一种芽孢荧光染色液及其应用
, , 1900 ,
本发明公开一种芽孢荧光染色液及其应用；本发明将荧光增白剂220用于检测芽孢；本发明的芽孢荧光染色液包括荧光增白剂220。本发明芽孢荧光染色液组分简单，成本低，能准确检测待测样本中的芽孢，且毒性低，对环境影响小；本发明的检测方法操作简便，测定快速，一步操作就能完成。
一种抗酸染色液制备容器
, , 1900 ,
本实用新型公开了一种抗酸染色液制备容器，涉及抗酸染色液制备领域，本实用新型包括容器主体，容器主体上通过密封轴承连接有搅拌轴，容器主体的顶部两侧均设有进料筒。本实用新型通过设置有进料组件，当需将液体定量进入至容器主体内部进行混合时，可将液体先加入至进料筒内，此时由于通孔与进料筒错位，转环可封堵进料筒，进而防止液体直接流入至容器主体内部，当液体加入到合适位置后，刻度线的设置可计量进入至容器主体内部的液体量，工作人员通过旋钮转动搅拌杆，搅拌杆则带动齿轮转动，使转环转动，当通孔与进料筒对齐时，此时液体便可通过通孔流入至容器主体内部，通过该方式可精确计量加入至容器主体内的液体量。
portfolio
projects
生物质基固体酸催化汽油烷基化脱硫的研究
Published:
烷基化脱硫主要采用固体酸催化汽油中的噻吩类硫化物与其本身含有的烯烃发生烷基化反应，生成相对分子量更大沸点更高的烷基噻吩，然后通过蒸馏的方法分离出高沸点硫化物以达到脱硫的目的，与加氢脱硫法相比具有明显的成本和保持辛烷值等方面的优势，因此在石化工业中具有良好的应用前景。生物质基固体酸具有可降解，催化效率高，易回收利用等优点日益受到人们的关注。本项目拟采用生物质基固体酸催化汽油烷基化脱硫反应，利用固体核磁共振、红外光谱等手段深入研究此类固体酸的表面酸性对催化裂化汽油烷基化脱硫反应的影响，揭示生物质基固体酸催化剂的酸性和烷基化脱硫活性之间的关联关系，并利用原位固体核磁共振和原位红外光谱实验包括骤冷技术捕捉烷基化脱硫反应中的吸附态、反应中间体和产物的信息，结合量化理论计算，阐明固体酸催化汽油烷基化脱硫的反应机理，从而为生物质基固体酸催化材料在烷基化脱硫中的应用提供理论依据。
葫芦[10]脲的分子识别及作为超分子纳米反应器的研究
Published:
作为新一代超分子主体，葫芦脲(CB[n])分子因其在水相中良好的分子识别性能而在诸多领域受到广泛的研究和应用。其中，葫芦[10]脲展示了有别于其它葫芦脲的特殊的识别特性。本课题拟进一步研究葫芦[10]脲的分子识别性质，并初步探讨葫芦[10]脲作为超分子纳米反应器的可行性。拟利用特殊客体与葫芦[10]脲形成包合物沉淀的性质，建立一种简单、高效、经济的纯化葫芦[10]脲的方法；通过核磁以及紫外/荧光光谱及X-射线单晶衍射等手段，重点研究葫芦[10]脲与环状主体分子的识别作用，理解包合作用对环状主体光电性质、识别性质及其构型的影响；通过考察水相环境中在葫芦[10]脲空腔内部发生的催化氧化、环化等反应(重点研究葫芦[10]脲与金属卟啉包合物的催化性能)，以期达到区域选择性加速/催化有机反应的效果。本项目对于扩展葫芦脲超分子化学以及发展绿色合成化学具有重要的科学意义和学术价值。
活细胞线粒体膜荧光标记及超分辨成像研究
Published:
线粒体是细胞内重要的细胞器，负责能量代谢、钙离子稳态、细胞凋亡等多种生理功能。对线粒体膜结构的精细研究，有助于深入了解线粒体在细胞生命活动中的作用机制。传统的光学显微镜由于衍射极限的限制，无法分辨小于200纳米的结构，而线粒体膜的厚度只有几纳米，因此，利用超分辨显微镜技术对线粒体膜进行超分辨率成像具有重要意义。
活细胞线粒体比例荧光超分辨成像研究
Published:
随着生命科学的快速发展，超分辨显微成像技术在生物医学成像领域表现出极大的应用与发展潜力，然而，超分辨成像在应用上还存在活细胞成像、定量信息获取及过程动态研究困难等问题。申请人拟发展一种基于比例荧光型的超分辨成像方法，设计适合于该方法的新型比例荧光探针，优化超分辨成像系统及升级重构软件，实现活细胞的超分辨比例成像及特定目标或动态过程的定量信息获取；将优化后的新型比例荧光探针用于特异性共价标记线粒体内、外膜蛋白质（腺嘌呤核苷转位酶与电压依赖阴离子通道蛋白），实现标记线粒体及其内外膜，利用比例荧光的超分辨成像方法对线粒体膜及蛋白质进行高分辨成像。通过重构软件，可以获取线粒体膜蛋白质相互作用、线粒体融合-分裂、网管化等动态过程的定量信息，研究其生物学功能。该成像方法的应用可从亚细胞甚至分子层面为线粒体相关疾病的发病机制研究提供定量信息，为精准医疗提供新的工具，推动生物医学深入发展，未见文献报道。
新型STORM成像材料的研究
Published:
新型STORM成像材料的研究意义在于，它直接突破了现有技术的瓶颈，推动生命科学进入一个更精细、更动态的观测维度。通过开发更亮、更稳定、毒性更低且具备优异光开关特性的荧光探针（如新型有机染料和功能化纳米颗粒），研究人员得以在活细胞中进行长时程、低损伤的超高分辨率成像，实时捕捉关键的生命动态过程。同时，这些新材料极大地提升了成像质量和分辨率，并拓展了多色成像的能力，使得科学家能够以前所未有的清晰度，在分子层面解析复杂生物结构的精细构造及其相互作用网络，从而深刻揭示生命的奥秘。
细胞成像系列AIE探针
Published:
近年来，随着生物分子学、分子影像学的发展，加上各种荧光探针被不断开发，人们对细胞的结构、形态研究表明其与疾病的发生和恶化有着密不可分的关系。而在生物体内的大平衡体系，各种亚细胞结构及细胞器内物种的浓度保持平衡但分布不尽相同。而且，细胞、亚细胞层次的离子及活性小分子物种的浓度变化或者细胞器形态的变化可能与疾病有着至关重要的联系。但是目前商品化的细胞成像探针主要是基于传统荧光染料分子，他们或多或少都面临着聚集诱导猝灭（ACQ）的问题，这些问题导致他们的成像质量存在各种问题，而 AIE 材料在光稳定性、抗光漂白性等方面拥有明显优于传统荧光染料的性质，因此，本项目拟基于 AIE 材料开发出适用于活细胞成像的荧光染料。该染料应该能够在主体功能上媲美目前现有的商品化染料，并且在一些方面具有比目前商品化探针更好的成像性能，如在长时间扫描过程中拥有更少的荧光损失、在高浓度工作下具有更低的细胞毒性等。争取用一年时间开发出一款具有完全知识产权的细胞成像探针，打破口碑和市场都一边倒向进口产品的尴尬局面。
白细胞五分类染色试剂的开发
Published:
血细胞分为红细胞(Red Blood Cell ,RBC)、白细胞(White Blood Cell ,WBC)、血小板(Platelet，PLT)三类细胞，血常规检查时经常需要检测白细胞分类及数量、红细胞数量和血小板数量。正常外周血中的白细胞通常可分为五类，即淋巴细胞、单核细胞、中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞。正常外周血中的红细胞包括成熟红细胞和少量网织红细胞(reticulocyte，RET)。目前基于核酸检测的全自动血细胞分析仪对血细胞分类检测主要在三个通道内进行的，分别是DIFF通道，RET通道和NRBC通道。主要利用半导体激光流式细胞术、核酸荧光染色技术对血细胞进行分类。其中DIFF四分群通道主要对白细胞进行分类检测，采用溶血剂完全溶解红细胞和血小板，白细胞膜仅部分溶解。核酸荧光染料进入白细胞内，使DNA、RNA和细胞器着色。因为荧光强度与细胞内核酸含量成比例，所以未成熟粒细胞、异常细胞荧光染色深，成熟白细胞荧光染色浅，从而得到DIFF（四分群）白细胞散点图；不同白细胞核酸含量有细微的差别，红细胞在逐渐发育成熟的过程中RNA含量逐渐减少直至消失。网织红细胞是红细胞的未成熟阶段，是反映骨髓红系造血功能以及判断贫血和相关疾病疗效的重要指标。骨髓中红细胞系统的增生发育过程是：多能干细胞→单能干细胞→原始红细胞→早幼红细胞→中幼红细胞→晚幼红细胞→网织红细胞→成熟红细胞。从原始红细胞增殖到晚幼红细胞阶段共分裂3-4次，约需72小时，红细胞数由一个变为8-16个，细胞核由大变小而浓缩，胞浆中含血红蛋白逐渐增多。晚幼红细胞以后细胞即不再分裂，发育过程中核被排出而成为网织红细胞。网织红细胞含有少量核糖核酸RNA，用煌焦油蓝染色时成网状故名网织红细胞。网织红细胞进一步成熟，RNA消失而为成熟红细胞。从晚幼红细胞发育到成熟红细胞约需48小时，成熟红细胞的寿命约为120天。在正常情况下骨髓中有核红细胞并不释放至血循环，只有网织红细胞和成熟红细胞才释入血中。因此，检查末梢血中网织红细胞数，可以推知骨髓生红细胞的情况。RET通道主要对红细胞进行分类检测，其检测机理在于荧光染料将未成熟红细胞中的大量残存核酸染色，用流式细胞术的原理进行检测。被荧光染色的网织红细胞根据荧光强度的强弱分类为四部分：HFR（高荧光强度网织红细胞比率）、MFR（中荧光强度网织红细胞比率）、LFR（低荧光强度网织红细胞比率）、RBC（红细胞）。分类为HFR、MFR、LFR的红细胞称为网织红细胞，特别是分类为HFR、MFR的网织红细胞作为IRF（未成熟网织红细胞指数）计数。另外，分类为RBC的红细胞作为成熟红细胞计数。采用AIE荧光核酸染色技术，利用AIE分子斯托克位移大的特点或许可以整合DIFF通道和RET通道，减少检测步骤，节约检测时间，提高检测效率。
AIE荧光探针的筛选和评价体系
Published:
AIE（Aggregation-Induced Emission，聚集诱导发光）分子作为一类由中国科学家原创并引领世界的明星分子，其在溶液中分散时荧光微弱，而在聚集状态下则发出强光的独特性质，彻底颠覆了传统发光材料的应用范式。开发AIE分子筛选及评价系统，其核心意义在于建立一个高通量、自动化、智能化的平台，以系统性地加速新型AIE分子的发现、性能优化与应用转化进程。该系统能够精准对接生物医药、光电器件、环境监测等领域的具体需求，通过快速、大规模地筛选和评价候选分子的光物理特性、生物相容性及靶向识别能力等关键参数，从而极大地缩短研发周期、降低科研成本，为开发高性能的生物探针、高效的OLED材料、高灵敏度的化学传感器乃至创新的诊疗一体化药物提供强大的技术支撑，是推动AIE这一优势领域从源头创新走向产业化应用的关键环节。
基于聚集诱导发光特性的新型融合蛋白标签技术
Published:
蛋白质特异性标记技术在生物学研究中的广泛应用背景，其中基于蛋白质标签特异性标记小分子的方法凭借标记的灵活性、特异性、稳定性和分子结构功能多样性等特点获得迅速发展，在活细胞内靶向蛋白质的实时动态研究中获得越来越多的关注，目前的标记方法所使用的传统荧光探针已经逐渐无法满足日益增长的实际要求，构建新的蛋白质特异性标记体系并进一步利用新型荧光功能分子实现特异性标记对于相关研究具有重要意义。
AIE药敏应用开发
Published:
AIE药敏应用开发项目针对当前全球日益严峻的细菌耐药性问题以及传统药敏检测耗时过长的临床痛点，提出了一种革命性的解决方案。该项目利用聚集诱导发光（Aggregation-Induced Emission, AIE）材料在特定条件下“从无到有”发光的独特优势，开发新型的荧光探针和检测平台，能够快速、灵敏地监测并区分出耐药菌与敏感菌。这有望将传统需要24至72小时的药敏检测时间缩短至数小时甚至更短，从而帮助医生第一时间为患者选择最有效的抗生素，避免因延误治疗导致的病情加重和经验性使用广谱抗生素所引发的耐药性进一步扩散。因此，该项目的成功不仅能极大地提升感染性疾病的精准诊疗水平、改善患者预后，更对遏制全球抗生素耐药性危机、保障公共卫生安全具有重大的战略价值和广阔的临床应用前景。
publications
Acid properties of solid acid from petroleum coke by chemical activation and sulfonation
Published in Catalysis Communincations, 2013
A novel solid acid was prepared from petroleum coke by KOH chemical activation and concentrated H2SO4 sulfonation. The solid acid was characterized by XRD, FT-IR and solid-state NMR. The characterization results show that the chemical activation and sulfonation lead to three functional Brønsted acid sites, –OH, –COOH and –SO3H on the solid acid. The probe molecules experimental reveal that the acid strength of the solid acid is stronger than that of SO42− /ZrO2, but slightly weaker than that of 100% H2SO4. The catalytic performance was evaluated by the esterification of oleic acid with methanol. The results indicate that this solid acid catalyst is very active, corresponding to high conversion (72%) of esterification reaction. In addition, the spent solid acid can be recovered by simple regeneration process.
Recommended citation: Zeng, D.; Liu, S.; Gong, W.; Wang, G.; Qiu, J.; Tian, Y. Acid Properties of Solid Acid from Petroleum Coke by Chemical Activation and Sulfonation. Catalysis Communications 2013, 40, 5–8. https://doi.org/10.1016/j.catcom.2013.05.018.
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Synthesis, characterization and acid catalysis of solid acid from peanut shell
Published in Applied Catalysis A: General, 2013
A strong Brønsted solid acid was synthesized by sulfonation of the partially carbonized agricultural biowaste peanut shell. The acidity of the Brønsted solid acid was characterized by X-ray diffraction (XRD), Fourier-transform infrared spectra (FT-IR) and solid-state nuclear magnetic resonance (NMR) spectroscopy. The characterization results show that sulfonation on the peanut shell carbon produces a carbon based solid acid containing three functional Brønsted acid sites, weak acidic single bondOH groups, strong acidic single bondCOOH and single bondSO3H groups. The acid strength of the solid acid is stronger than that of HZSM-5(Si/Al = 75), but still weaker than that of 100% H2SO4. The catalytic reaction tests indicate that this solid acid catalyst exhibits high activity and excellent recyclability for biodiesel production.
Recommended citation: Zeng, D.; Liu, S.; Gong, W.; Wang, G.; Qiu, J.; Chen, H. Synthesis, Characterization and Acid Catalysis of Solid Acid from Peanut Shell. Applied Catalysis A: General 2014, 469, 284–289. https://doi.org/10.1016/j.apcata.2013.09.038.
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A Brønsted solid acid synthesized from fly ash for vapor phase dehydration of methanol
Published in Fuel, 2013
A Brønsted solid acid was prepared from waste coal fly ash by acid treatment with concentrated H2SO4 at 473 K. The prepared solid acid was characterized by X-ray diffraction (XRD), Fourier-transform Infrared spectra (FT-IR), solid-state nuclear magnetic resonance (NMR) and scanning electron microscope (SEM). The characterization results show that the solid acid contains two types of Brønsted acid sites, weak acidic –OH groups and strong bridging Si–O(H)–Al groups. The acid strength of the strong acid sites is similar to that of zeolite HY (Si/Al = 3). The solid acid from fly ash exhibits excellent catalytic activity and stability in methanol dehydration reaction for dimethyl ether (DME) production. Our finding is a promising way of utilization of waste coal fly ash to prepare effective solid acid catalyst for industrial acid catalysis reactions.
Recommended citation: Zeng, D.; Liu, S.; Gong, W.; Qiu, J.; Chen, H.; Wang, G. A Brønsted Solid Acid Synthesized from Fly Ash for Vapor Phase Dehydration of Methanol. Fuel 2014, 119, 202–206. https://doi.org/10.1016/j.fuel.2013.11.030.
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Self-Healing Supramolecular Polymers via Host-Guest Interactions
Published in Current Organic Chemistry, 2014
Self-healing or self-repairing materials are ‘smart materials’ that repair damage caused by mechanical force and are a key development of 21st century materials chemistry and engineering. In this review we describe a few excellent examples of self-healing supramolecular polymers based on host-guest interactions and discuss their advantages and versatility.
Recommended citation: Liu S.; Gong, W.;Yang X. Self-Healing Supramolecular Polymers via Host-Guest Interactions Current Organic Chemistry 2014, 18 (15), 2010 - 2015. https://doi.org/10.2174/1385272819666140514005435.
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利用转炉尘泥制备聚合硫酸铁的研究
Published in 现代化工, 2014
以转炉尘泥为原料,采用混合酸酸浸法浸出铁,探讨了硫酸浓度、反应温度、反应时间、盐酸用量对铁浸出率的影响。将浸出液经铁粉还原、双氧水氧化工艺制备聚合硫酸铁,并对制得的聚合硫酸铁进行了一系列的分析和表征。实验结果表明,硫酸质量分数为70%,反应温度为80℃,反应时间为120 min,盐酸用量为15 mL时,铁的浸出率可达95%以上
Recommended citation: 曾丹林 ;刘胜兰 ;王光辉; 马亚丽; 苏敏; 龚晚君; 严铁军,利用转炉尘泥制备聚合硫酸铁的研究.现代化工, 2013, 3(12):67-69.DOI:10.16606/j.cnki.issn0253-4320.2013.12.033.
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A nano-sized solid acid synthesized from rice hull ash for biodiesel production
Published in RSC Advances, 2014
A nano-sized solid acid was synthesized from rice hull ash by acid activation. The solid acid was characterized by XRD, FT-IR, TEM and solid-state NMR spectroscopy. The characterization results show that the solid acid is amorphous silica with –OH and –SO3H functional acid groups. The TEM images show that the particle size range of the solid acid catalyst is 50–100 nm. In addition, the catalytic results indicate that the solid acid exhibits excellent activity and recyclability for the transesterification reaction of soybean oil with methanol, suggesting promising industrial applications in biodiesel production.
Recommended citation: Zeng, D.; Liu, S.; Gong, W.; Chen, H.; Wang, G. A Nano-Sized Solid Acid Synthesized from Rice Hull Ash for Biodiesel Production. RSC Adv. 2014, 4 (39), 20535–20539. https://doi.org/10.1039/C4RA00266K.
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Effect of surface properties of iron oxide sorbents on hydrogen sulfide removal from odor
Published in Clean - Soil, Air, Water, 2015
Hydrogen sulfide (H2S) removal is the key issue for the odor control of sewage treatment facilities. In this paper, the iron oxide sorbents with different surface acid–base properties were prepared by acid and base modification. Effect of surface properties of iron oxide sorbents on H2S removal was revealed by the characterization of surface pH, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and solid-state NMR spectroscopy. The results show that the activity loss of the iron oxide sorbent is mainly due to the gradual oxidation of H2S into elemental sulfur and further into the sulfuric acid and sulfurous acid with time on stream. Meanwhile, the surface basic environment of base-treated iron oxide sorbent significantly increases the H2S removal activity by suppression the formation of H2SO4 or H2SO3 during the desulfurization process. Our findings will help us to prepare novel sorbent for more effective H2S removal from, e.g., odor gas.
Recommended citation: Zeng, D.; Liu, S.; Gong, W.; Wang, G.; Qiu, J.; Chen, H. Effect of Surface Properties of Iron Oxide Sorbents on Hydrogen Sulfide Removal from Odor. CLEAN Soil Air Water 2015, 43 (7), 975–979. https://doi.org/10.1002/clen.201300328.
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Cucurbituril-based supramolecular nanoreactors/catalysts
Published in Progress in Chemistry, 2016
Cucurbit[n] urils (CB[n] s), as a kind of rapidly developing supramolecular hosts, have been attracting more and more attentions. With their rigid structures, hydrophobic cavities and electronegative carbonyl groups on the portals, CB[n] s show their unique recognition properties——high selectivity and high binding affinity toward organic cations. In the past decade, CB[n] s have been utilized not only in basic recognition research but also in the construction of complicated three-dimensional materials and even in drug delivery systems. Besides, CB[n] s have been creatively used to control the reaction process and have obtained numerous successes. Herein, this review mainly describes the use of cucurbiturils as supramolecular nanoreactors/catalysts to accelerate or control the reaction process in thermal reactions as well as photoreactions. Moreover, the inhibition effect on guest's activity caused by the encapsulation inside cucurbiturils is also discussed.
Recommended citation: Gong W.; Zhao Z.; Liu S.; Cucurbituril-based supramolecular nanoreactors/catalysts. Progress in Chemistry, 2016, 28 (12): 1732-1742. https://doi.org/10.7536/PC160936
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From Packed “Sandwich” to “Russian Doll”: Assembly by Charge-Transfer Interactions in Cucurbit[10]uril
Published in Chemistry - A European Journal, 2016
Invited for the cover of this issue is the group of Lyle Isaacs and Simin Liu at the University of Maryland and Wuhan University of Science and Technology. The image depicts two packed “sandwiches” and one supramolecular “Russian doll” that are assembled by host–guest and charge-transfer interactions.
Recommended citation: Gong, W.; Yang, X.; Zavalij, P. Y.; Isaacs, L.; Zhao, Z.; Liu, S. From Packed “Sandwich” to “Russian Doll”: Assembly by Charge‐Transfer Interactions in Cucurbit[10]Uril. Chemistry A European J, 2016, 22 (49), 17493–17493. https://doi.org/10.1002/chem.201605040.
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From Packed “Sandwich” to “Russian Doll”: Assembly by Charge-Transfer Interactions in Cucurbit[10]uril
Published in Chemistry - A European Journal, 2016
As the host possessing the largest cavity in the cucurbit[n]uril (CB[n]) family, CB[10] has previously displayed unusual recognition and assembly properties with guests but much remains to be explored. Herein, we present the recognition properties of CB[10] toward a series of bipyridinium guests including the tetracationic cyclophane known as blue box along with electron-rich guests and detail the influence of encapsulation on the charge-transfer interactions between guests. For the mono-bipyridinium guest (methylviologen, MV2+), CB[10] not only forms 1:1 and 1:2 inclusion complexes, but also enhances the charge-transfer interactions between methylviologen and dihydroxynaphthalene (HN) by mainly forming the 1:2:1 packed “sandwich” complex (CB[10]⋅2 MV2+⋅HN). For guest 1 with two bipyridinium units, an interesting conformational switching from linear to “U” shape is observed by adding catechol to the solution of CB[10] and the guest. For the tetracationic cyclophane-blue box, CB[10] forms a stable 1:1 inclusion complex; the two bipyridinium units tilt inside the cavity of CB[10] according to the X-ray crystal structure. Finally, a supramolecular “Russian doll” was built up by threading a guest through the cavities of both blue box and CB[10].
Recommended citation: Gong, W.; Yang, X.; Zavalij, P. Y.; Isaacs, L.; Zhao, Z.; Liu, S. From Packed “Sandwich” to “Russian Doll”: Assembly by Charge‐Transfer Interactions in Cucurbit[10]Uril. Chemistry A European J 2016, 22 (49), 17493–17493. https://doi.org/10.1002/chem.201604149.
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Inhibition and Stabilization: Cucurbituril Induced Distinct Effects on the Schiff Base Reaction
Published in Journal of Organic Chemistry, 2017
The different effects of cucurbit[7]uril (CB[7]) on the Schiff base reactions in aqueous solution were explored by 1H NMR spectroscopy and single X-ray crystallography. With CB[7], the condensation reaction of aldehyde and primary amine is dramatically inhibited. In contrast, the presence of CB[7] does tremendously stabilize iminium cation in water through ion–dipole interactions. A single crystal structure of the complex of iminium ion 7 with CB[7] grown in water is reported.
Recommended citation: Gong, W.; Ma, J.; Zhao, Z.; Gao, F.; Liang, F.; Zhang, H.; Liu, S. Inhibition and Stabilization: Cucurbituril Induced Distinct Effects on the Schiff Base Reaction. J. Org. Chem., 2017, 82 (6), 3298–3301. https://doi.org/10.1021/acs.joc.6b02971.
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Fluorescence lifetime imaging of microviscosity changes during ER autophagy in live cells
Published in Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2018
Unfolded or misfolded protein accumulation inside Endoplasmic Reticulum (ER) will cause ER stress and subsequently will activate cellular autophagy to release ER stress, which would ultimately result in microviscosity changes. However, even though, it is highly significant to gain a quantitative assessment of microviscosity changes during ER autophagy to study ER stress and autophagy behaviors related diseases, it has rarely been reported yet. In this work, we have reported a BODIPY based fluorescent molecular rotor that can covalently bind with vicinal dithiols containing nascent proteins in ER and hence can result in ER stress through the inhibition of the folding of nascent proteins. The change in local viscosity, caused by the release of the stress in cells through autophagy, was quantified by the probe using fluorescence lifetime imaging. This work basically demonstrates the possibility of introducing synthetic chemical probe as a promising tool to diagnose ER-viscosity-related diseases.
Recommended citation: He, Y.; Qu, J.; Samanta, S.; Liu, W.; Pan, W.; Gong, W.; Yang, Z. Fluorescence Lifetime Imaging of Microviscosity Changes during ER Autophagy in Live Cells. In Biophotonics and Immune Responses XIII; Chen, W. R., Ed.; SPIE: San Francisco, United States, 2018; p 26. https://doi.org/10.1117/12.2293440.
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Organic fluorescent probes for stochastic optical reconstruction microscopy (STORM): Recent highlights and future possibilities
Published in Coordination Chemistry Reviews, 2018
Super-resolution fluorescence imaging by single-molecule localization microscopy (SMLM) offers the possibility of microscopic images with sub-diffraction spatial resolution. Stochastic optical reconstruction microscopy (STORM) is one of the emerging SMLM techniques that has contributed new insights into both the structures and functions of sub-cellular organelles in the cellular context with a spatial resolution virtually at the molecular level. Photo-switching of single fluorophores and position determination are the most common features of this SMLM technique, which allows molecule-resolved information as well as super-resolved images. However, achieving successful STORM-based images relies on the suitable choice of a fluorophore. In particular, the use of ideal organic fluorescent probes has great potential to circumvent common difficulties that arise during the construction of STORM images. However, there is hardly any comprehensive review that critically assesses the criteria for choosing ideal fluorescent probes for STORM and designing new efficient organic fluorescent probes to date. Therefore, this review has particularly focused on the choice of organic fluorescent probes, the essential features for designing new probes and the future prospects for resolving persistent issues in STORM imaging. The utility of organic fluorescent probes in multicolor STORM, 3D STORM and live cell STORM imaging are also discussed to provide a perspective concerning the true application potential of commonly used fluorescent dyes. In this review, we not only describe how organic fluorescent dyes have contributed to the growth of STORM-based super-resolution imaging in eukaryotic biology, but we also attempt to provide a basis on which advanced organic fluorescent probes can be designed and developed in the near future.
Recommended citation: Samanta, S#.; Gong, W. #; Li, W.; Sharma, A.; Shim, I.; Zhang, W.; Das, P.; Pan, W.; Liu, L.; Yang, Z.; Qu, J.; Kim, J. S. Organic Fluorescent Probes for Stochastic Optical Reconstruction Microscopy (STORM): Recent Highlights and Future Possibilities. Coordination Chemistry Reviews 2019, 380, 17–34. https://doi.org/10.1016/j.ccr.2018.08.006.
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Dual-functional fluorescent molecular rotor for endoplasmic reticulum microviscosity imaging during reticulophagy
Published in Chemical Communications, 2019
The microviscosity change associated with reticulophagy is an important component for studying endoplasmic reticulum (ER) stress disorders. Here, a BODIPY-arsenicate conjugate 1-based fluorescent molecular rotor was designed to covalently bind vicinal dithiol-containing proteins in the ER, exhibiting a bifunction of reticulophagy initiation and microviscosity evaluation. Therefore, we could quantify the local viscosity changes during reticulophagy based on the fluorescence lifetime changes of probe 1.
Recommended citation: He, Y.; Shin, J.; Gong, W.; Das, P.; Qu, J.; Yang, Z.; Liu, W.; Kang, C.; Qu, J.; Kim, J. S. Dual-Functional Fluorescent Molecular Rotor for Endoplasmic Reticulum Microviscosity Imaging during Reticulophagy. Chem. Commun. 2019, 55 (17), 2453–2456. https://doi.org/10.1039/C9CC00300B.
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Redefining the photo-stability of common fluorophores with triplet state quenchers: Mechanistic insights and recent updates
Published in Chemical Communications, 2019
Light microscopy can offer certain advantages over electron microscopy in terms of acquiring detailed insights into the biological/intra-cellular milieu. In recent years, with the development of new fluorescence imaging technologies, it has become extremely important to assess the role of designing appropriate fluorophores in acquiring desired biological information without encountering any untoward hitches. Over the years, external fluorophores have been prevalently used in fluorescence microscopy and single-molecule fluorescence microscopy-based studies. Photostable fluorogenic probes with high extinction coefficients and quantum yields, exhibiting minimum autofluorescence and photobleaching properties, are preferred in single-molecule microscopy as they can tolerate long-term laser exposure. Therefore, the development of triplet state quenchers and/or any other suitable new strategy to ensure the photo-stability of the fluorophores during long-term live cell imaging exercises is highly anticipated. In this feature article, various strategies for stabilizing fluorophores, including the mechanisms of TSQ-induced stabilization, have been thoroughly reviewed considering contemporary literature reports and applications.
Recommended citation: Gong, W.; Das, P.; Samanta, S.; Xiong, J.; Pan, W.; Gu, Z.; Zhang, J.; Qu, J.; Yang, Z. Redefining the Photo-Stability of Common Fluorophores with Triplet State Quenchers: Mechanistic Insights and Recent Updates. Chem. Commun., 2019, 55 (60), 8695–8704. https://doi.org/10.1039/C9CC02616A.
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Nanoliposomes Co-Encapsulating Photoswitchable Probe and Photosensitizer for Super-Resolution Optical Imaging and Photodynamic Therapy
Published in Cytometry Part A, 2019
Photosensitizers (PSs) are ideal cancer theranostic drugs that can be administered as both fluorescence imaging reagents and photodynamic therapy (PDT) drugs. To improve the tumoritropic behavior of PSs, nanoliposomes are presently being considered as optimal PSs carriers. Although nanoliposomal PSs have been utilized in clinical therapy, PSs localization and photosensitive processing in nanoliposomal PSs are rarely observed on nanoscale. Investigating changes in the fine structure of nanoliposomes under photosensitive processing will further our understanding of the photosensitive effect on nanoliposomal PSs. In this study, nanoliposomes co-encapsulating the PSs benzoporphyrin derivative monoacid A (BPD) and the photoswitchable probe Cy5-927 were prepared to realize PDT and nanoscale super-resolution optical imaging. The fine structures of nanoliposomal BPD and Cy5-927 (LBC) were visualized by a home-built stochastic optical reconstruction microscopy (STORM). Our PDT results showed that the photorelease and PDT efficiency of BPD were not decreased by co-encapsulating with Cy5-927 in LBC. Taken together, LBC can be used as a new optical probe and PDT reagent for investigating changes in nanoliposomes fine structure and micro-interaction in the cellular process of PDT. Therefore, our results deepened our understanding of liposome-based PDT for optimizing cancer treatment.
Recommended citation: Xu, H.#; Chen, B.#; Gong, W.#; Yang, Z.; Qu, J. Nanoliposomes Co‐Encapsulating Photoswitchable Probe and Photosensitizer for Super‐Resolution Optical Imaging and Photodynamic Therapy. Cytometry Pt A 2020, 97 (1), 54–60. https://doi.org/10.1002/cyto.a.23864.
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Super-resolution imaging of the dynamic cleavage of intercellular tunneling nanotubes
Published in Frontiers of Optoelectronics, 2020
As a new method of cell-cell communication, tunneling nanotubes (TNTs) play important roles in cell-cell signaling and mass exchanges. However, a lack of powerful tools to visualize dynamic TNTs with high temporal/spatial resolution restricts the exploration of their formation and cleavage, hindering the complete understanding of its mechanism. Herein, we present the first example of using stochastic optical reconstruction microscopy (STORM) to observe the tube-like structures of TNTs linking live cells with an easily prepared fluorescent dye. Because of this new imaging microscopy, the cleavage process of TNTs was observed with a high spatial resolution.
Recommended citation: Gong, W.; Pan, W.; He, Y.; Huang, M.; Zhang, J.; Gu, Z.; Zhang, D.; Yang, Z.; Qu, J. Super-Resolution Imaging of the Dynamic Cleavage of Intercellular Tunneling Nanotubes. Front. Optoelectron., 2020, 13 (4), 318–326. https://doi.org/10.1007/s12200-020-1068-1.
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STORM imaging of mitochondrial dynamics using a vicinal-dithiol-proteins-targeted probe
Published in Biomaterials, 2020
Stochastic optical reconstruction microscopy (STORM) is a promising method for the visualization of ultra-fine mitochondrial structures. However, this approach is limited to monitoring dynamic intracellular events owing to its low temporal resolution. We developed a new strategy to capture mitochondrial dynamics using a compressed sensing STORM algorithm following raw data pre-treatments by a noise-corrected principal component analysis and K-factor image factorization. Using STORM microscopy with a vicinal-dithiol-proteins targeting probe, visualizing mitochondrial dynamics was attainable with spatial and temporal resolutions of 45 nm and 0.8 s, notably, dynamic mitochondrial tubulation retraction of ~746 nm in 1.2 s was monitored. The labeled conjugate was observed as clusters (radii, ~90 nm) distributed on the outer mitochondrial membranes, not yet reported as far as we know. This strategy is promising for the quantitative analysis of intracellular behaviors below the optical diffraction limit.
Recommended citation: Chen, B.#; Gong, W.#; Yang, Z.; Pan, W.; Verwilst, P.; Shin, J.; Yan, W.; Liu, L.; Qu, J.; Kim, J. S. STORM Imaging of Mitochondrial Dynamics Using a Vicinal-Dithiol-Proteins-Targeted Probe. Biomaterials, 2020, 243, 119938. https://doi.org/10.1016/j.biomaterials.2020.119938.
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Gas probes and their application in gas therapy
Published in Chemical Synthesis, 2021
As an emerging field, gas therapy attracts increasing attention because of its distinguishing features in disease treatment. However, to achieve a therapeutic effect, the concentration of gas should be carefully controlled. Thus, a suitable and convenient technology is required to monitor the gas concentration in vivo. Besides, the transportation of gas into human body and in vivo biodistribution of gas also need to be evaluated. Among the technologies adopted in gas therapy, fluorescence imaging technology is the first choice due to its high specificity, high sensitivity, and non-invasion. And as the core of fluorescence imaging, the properties of fluorescent dyes directly determine the quality of imaging. So, it is critical to choose suitable gas probes for different purposes. Here, we review common gas detection methods, including a brief introduction of fluorescence, the distinctive properties of five fluorophore cores, and the detection mechanisms of common gas probes. Then, the applications of gas probes in gas delivery, gas release, and gas therapy are summarized. At last, we discuss the potential of developing further intelligent gas probes and fluorescence imaging technologies for gas therapy.
Recommended citation: Gong, W.-J.; Yu, Z.-Q.; He, Q.-J. Gas Probes and Their Application in Gas Therapy. Chem. Syth., 2021. https://doi.org/10.20517/cs.2021.04.
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Sulourea-coordinated Pd nanocubes for NIR-responsive photothermal/H2S therapy of cancer
Published in Journal of Nanobiotechnology, 2021
The Pd-Su nanomedicine shows a high Su loading capacity (85 mg g−1), a high near-infrared (NIR) photothermal conversion efficiency (69.4%), and NIR-controlled H2S release by the photothermal-triggered hydrolysis of Su. The combination of photothermal heating and H2S produces a strong synergetic effect by H2S-induced inhibition of heat shock response, thereby effectively inhibiting tumor growth. Moreover, high intratumoral accumulation of the Pd-Su nanomedicine after intravenous injection also enables photothermal/photoacoustic dual-mode imaging-guided tumor treatment.
Recommended citation: Guo, X.; Liu, J.; Jiang, L.; Gong, W.; Wu, H.; He, Q. Sulourea-Coordinated Pd Nanocubes for NIR-Responsive Photothermal/H2S Therapy of Cancer. J Nanobiotechnol, 2021, 19 (1), 321. https://doi.org/10.1186/s12951-021-01042-9.
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An Activity-Based Ratiometric Fluorescent Probe for In Vivo Real-Time Imaging of Hydrogen Molecules
Published in Angewandte Chemie - International Edition, 2022
To reveal the biomedical effects and mechanisms of hydrogen molecules urgently needs hydrogen molecular imaging probes as an imperative tool, but the development of these probes is extremely challenging. A catalytic hydrogenation strategy is proposed to design and synthesize a ratiometric fluorescent probe by encapsulating Pd nanoparticles and conjugating azido-/coumarin-modified fluorophore into mesoporous silica nanoparticles, realizing in vitro and in vivo fluorescence imaging of hydrogen molecules. The developed hydrogen probe exhibits high sensitivity, rapid responsivity, high selectivity and low detection limit, enabling rapid and real-time detection of hydrogen molecules both in cells and in the body of animal and plant. By application of the developed fluorescent probe, we have directly observed the super-high transmembrane and ultrafast transport abilities of hydrogen molecules in cells, animals and plants, and discovered in vivo high diffusion of hydrogen molecules.
Recommended citation: Gong, W.; Jiang, L.; Zhu, Y.; Jiang, M.; Chen, D.; Jin, Z.; Qin, S.; Yu, Z.; He, Q. An Activity‐Based Ratiometric Fluorescent Probe for In Vivo Real‐Time Imaging of Hydrogen Molecules. Angew. Chem. Int. Ed., 2022, 61 (9), e202114594. https://doi.org/10.1002/anie.202114594.
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Fe-porphyrin: A redox-related biosensor of hydrogen molecule
Published in Nano Research, 2022
Hydrogen molecule (H2) exhibits broad-spectrum but microenvironment-dependent biomedical effects in varied oxidation stress-related diseases, but its molecular mechanism is unclear and its targeting molecule is unknown so far. Herein, we originally reveal that Fe-porphyrin is a H2-targeted molecule. We have demonstrated that the oxidized Fe-porphyrin in both free and protein-confining states can self-catalyze the hydrogenation/reduction by reacting with H2 to catalytically scavenge ·OH, and can also catalytically hydrogenate to reduce CO2 into CO in the hypoxic microenvironment of in vitro simulation and in vivo tumor, confirming that Fe-porphyrin is a redox-related biosensor of H2 and H2 is an upstream signaling molecule of CO. These discoveries are favorable for deep understanding and exploration of profound biomedical effects of H2, and helpful for development of innovative drugs and hydrogen energy/agricultural materials.
Recommended citation: Jin, Z.; Zhao, P.; Gong, W.; Ding, W.; He, Q. Fe-Porphyrin: A Redox-Related Biosensor of Hydrogen Molecule. Nano Res. 2023, 16 (2), 2020–2025. https://doi.org/10.1007/s12274-022-4860-y.
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ASBase: The universal database for aggregate science
Published in Aggregate, 2022
This paper reports the first universal and versatile database on aggregate materials for the field of aggregate science research. At the current stage, the database contains over 1000 entries of organic aggregate material systems (mainly luminescent systems at the current stage) with a unique data structure which is designed particularly for aggregate materials and containing the photophysics and physicochemical properties of the compounds in different statuses of aggregation, including dilute solution form, pristine solid-state, stable crystalline, and nanoaggregates formed in solvents. The web-based interface of the database provided functions to index, search, manipulate, fetch and deposit data entries. In addition, a background calculation service optimizes the chemical structure of new entries on different levels of accuracies. The database also provided background API for interactive developments of prediction or regression models based on machine-learning algorithms.
Recommended citation: Gong, J.; Gong, W.; Wu, B.; Wang, H.; He, W.; Dai, Z.; Li, Y.; Liu, Y.; Wang, Z.; Tuo, X.; Lam, J. W. Y.; Qiu, Z.; Zhao, Z.; Tang, B. Z. ASBase: The Universal Database for Aggregate Science. Aggregate, 2023, 4 (1), e263. https://doi.org/10.1002/agt2.263.
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聚集诱导发光材料：生物医学成像中的新视野
Published in 科学, 2024
本文介绍了聚集诱导发光（AIE）材料在生物医学成像领域的应用，强调了其在细胞器成像、微生物检测、肿瘤手术导航及小分子探测等方面的独特优势。AIE材料“越聚集越亮”的特性显著提升了生物检测的灵敏度和特异性，为精准医疗和疾病早期诊断提供了新的技术手段。
Recommended citation: Gong,W.; Liu, Y.; Wang, Z.; 聚集诱导发光材料：生物医学成像中的新视野. 科学 2024, 76 (05), 9–14.
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AIE生物成像图谱及技术手册
Published in 科学出版社, 2025
本书是一本聚焦前沿光学材料应用的跨学科工具书，系统呈现了“聚集诱导发光”（aggregation-induced emissin，AIE）材料在生命科学领域的创新突破与实践成果。全书通过三大模块构建知识体系：基础理论部分阐释了AIE材料的发光机制与特性优势以及荧光成像技术的广泛应用场景；图谱部分详细展示了AIE材料在不同层次的生物成像实例，涵盖生物大分子、细胞器、细胞及活体；常见问题部分针对荧光成像中的常见问题给出原因分析及解决方案，可助力初入荧光成像领域的基础及临床工作者快速了解相关知识。全书收录超过500幅技术原理及高清显微图像，直观呈现了AIE材料在不同环境及应用场景中的高信噪比、高靶向性、高稳定性及高穿透性等技术优势。成像图例提供了对应的AIE分子结构及详细的成像参数，为读者提供全面的技术参考。作为材料科学与生物医学的交叉研究成果，本书既可作为高校“生物医学工程”专业研究生教材，也可为相关生物技术企业研发部门提供技术转化指南，同时也能成为生物、医学等工作者的工具书。.
Organelle-Targeted AIE Probes for Cell Sorting
Published in Encyclopedia of Aggregation-Induced Emission, 2025
In conclusion, the future of AIE organelle-targeted probes in cell sorting is bright. As research continues to advance, we can expect to see significant improvements in probe design, integration with other technologies, and clinical applications. These advancements will have a profound impact on various fields, including cell biology, medicine, and biotechnology.
Recommended citation: Zhang, Y., Gong, W</em>. (2025). Organelle-Targeted AIE Probes for Cell Sorting. In: Tang, B.Z., Zhao, Z., Qiu, Z. (eds) Encyclopedia of Aggregation-Induced Emission. Springer, Singapore. https://doi.org/10.1007/978-981-97-1574-9_134-1 Published:
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talks
全球领先的聚集诱导发光荧光造影
基于 AIE 技术的微生物快速检测整体解决方案
从成像到预测FLI+AI在恶性肿瘤检测中的应用
全球领先的聚集诱导发光荧光造影
基于AIE技术的微生物快速检测整体解决方案
全球领先的聚集诱导发光荧光造影
聚集诱导发光高等研究院聚集诱导发光荧光纳米颗粒
基于AIE技术的微生物快速检测整体解决方案
聚集诱导发光材料在微生物检测中的应用
聚集诱导发光材料在微生物检测中的应用
聚集诱导发光材料在微生物检测中的应用
聚集诱导发光材料在微生物检测中的应用
聚集诱导发光材料在微生物检测中的应用
新型AIE聚集诱导发光材料用于血液白细胞分类
teaching